Another episode features Brain's single day where he tries to do anything but take over the world: in the end, a group of people votes that he should take over the world on the one day that he does not want to. One centers on Snowball's plan to take over the world using Microsponge (a parody of Microsoft). The bulk of every episode involves one of Brain's plans for world domination with Pinky's assistance and the ultimate failure of that plan, with some exceptions.

There is very little continuity between episodes outside of the common fixtures of the two mice, though some plans for world domination from early episodes are subsequently referred to in later seasons for example, Brain's mechanical human suit that was first used in 'Win Big' reappears when Brain faces his rival Snowball in 'Snowball'. Wells, Ivan Pavlov, and Johannes Gutenberg. Several episodes take place in historical times, with Pinky and Brain in the laboratory of some scientifically-minded person, including Merlin, H.G. Many of the Pinky and the Brain episodes occur in the 1990s at Acme Labs, located in some large American city underneath a suspension bridge. 7.1 On Animaniacs and Pinky and the Brain.In common with many other Animaniacs shorts, many episodes are in some way a parody of something else, usually a film or novel. In each episode, Brain devises a new plan to take over the world which ultimately ends in failure, usually due to the impossibility of Brain's plan, Brain's own arrogance or overconfidence, Pinky's bumbling, something Brain didn’t consider, circumstances beyond their control, or multiples thereof. Brain is highly intelligent, self-centered and scheming, while Pinky is good-natured but feeble-minded. Pinky and the Brain are genetically enhanced laboratory mice who reside in a cage in the Acme Labs research facility. The characters later appeared in the series Pinky, Elmyra & the Brain, and have since returned to their roots as an Animaniacs skit in the 2020 revival of that series. It was later spun off as a series due to its popularity, with 66 episodes produced.

The characters first appeared in 1993 as a recurring skit on Animaniacs. It was the first animated television series to be presented in Dolby Surround and the fourth collaboration of Steven Spielberg with his production company, Amblin Entertainment, and Warner Bros. Pinky and the Brain is an American animated television series that was created by Tom Ruegger that premiered on Kids' WB on September 9, 1995.

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